Carbon Quantum Dots (CQDs) hold promise in drug delivery due to their controlled release, targeting, and real-time monitoring capabilities driven by their biocompatibility and fluorescence properties. The synthesis of CQDs from watermelon seeds represents a highly advantageous approach from economic, environmental, and technological perspectives. This enables the development of advanced biomedical applications, such as precise drug delivery, contributing to sustainability and innovation in medicine. The methodology is divided into three phases: synthesis and characterization of CQDs, preparation of hydrogels, and drug release assays using CQDs via hydrogels. CQDs were synthesized via the hydrothermal method at 200°C for 10 hours, incorporating three different maturity levels. To comprehensively characterize the CQDs, a suite of analytical techniques was employed, including UV-visible spectroscopy, Raman spectroscopy, scanning electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy, and fluorescence spectroscopy. Polyvinyl alcohol (PVA) and Hydroxypropyl Methyl Cellulose (HPMC) are utilized in hydrogel formulation. PVA is homogenized at 140°C for 2 hours, while HPMC is homogenized at room temperature. Both hydrogels have a 6% concentration and use CQD solutions at 0.03 g/mol and 0.02 g/mol. To assess drug release, an initial cytotoxicity test was conducted employing the SH-SY5Y cell line. Analysis using UV-vis spectroscopy indicated that CQDs synthesized at maturity level II displayed optimal properties. In the Raman results, two predominant peaks are observed at approximately 1340 and 1590, commonly attributed to the disordered D band and crystalline G band, respectively. They exhibited nearly spherical morphology (SEM) with uniform agglomerations at room temperature. Furthermore, these CQDs demonstrated excellent water dispersibility, forming stable agglomerates, while retaining strong photoluminescence and displaying low cytotoxicity. CQDs' potent photoluminescence and low cytotoxicity make them excellent candidates for drug delivery via hydrogels, with potential for personalized medicine. Yet, challenges include drug loading optimization, precise targeting, and long-term safety assurance.