Ecuador is one of the most megadiverse countries in the world, hosting abundant flora with potential properties in the pharmaceutical, agricultural, and environmental industries. This study comprises a comprehensive biochemical analysis of four plant species: Juglans Neotropical, Duranta Triacantha, Mikania Sp, and Brugmansia Aurea. The content of bioactive compounds, antioxidant activity, and cytotoxicity were quantitatively evaluated. In turn, the results obtained from the methanolic extracts were compared with those obtained through the basic and acid hydrolysis of the supernatant resulting from the extraction. The methods used were Total flavonoid content (TFC), Total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), Ferric ion reducing antioxidant power (FRAP) and brine shrimp in cytotoxicity. The results indicated that the four species have a high content of phenols, flavonoids, and antioxidant capacity, with Juglans Neotropical leading with values obtained for TFC: 292 mg QE/ gDE, TPC: 270 mg GAE/ DE, DPPH: 517 ug/mL IC50, ABTS: 182 ug/mL IC50 and FRAP: 3.74 %FRA using Trolox as standard. Finally, it is non-toxic in concentrations less than 1 mg/mL. The present study suggests that the studied species present a favorable biochemical profile for further research.