We present a systematic revision of Pristimantis katoptroides integrating molecular, morphological, and biogeographic evidence. Our analysis reveals that P. katoptroides represents a complex of five distinct species, three of which are described herein: Pristimantis betancourt sp. nov., P. nua sp. nov., and P. warmi sp. nov, all distributed within a ~100 km of each other in the Cóndor–Kutukú mountain complex of the Santiago River basin. Although these taxa share a green, spiny phenotype, they can be distinguished by consistent differences in groin and ventral coloration as well as digital morphology. P. betancourt sp. nov. is characterized by a green venter with irregular white blotches, lavender-blue groins and tarsi in males, and lateral fringes on fingers and toes. P. nua sp. nov. has a pinkish-white venter, black groins in females, washed blue in males, and narrow lateral fringes. P. warmi sp. nov. presents a smoky-white venter with brown markings, indigo-blue groins in females, and an additional orange spot in males. In contrast, P. katoptroides sensu stricto exhibits a homogeneously white venter, with groin coloration ranging from smalt blue in males to washed pink in females, and is broadly distributed from the central Andes of Ecuador to the Colombian border in the Putumayo River basin. The Amazonian Mountain ranges adjacent to the eastern Andes, particularly the Santiago River basin in southeastern Ecuador, may represent the biogeographic origin of this clade. An additional lineage (Unconfirmed Candidate Species 1) is morphologically similar to P. betancourt sp. nov. but requires further molecular and morphological data for formal description